期刊
JOURNAL OF BACTERIOLOGY
卷 182, 期 8, 页码 2350-2353出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.182.8.2350-2353.2000
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资金
- NIAID NIH HHS [R01AI34968] Funding Source: Medline
- NIDDK NIH HHS [F32 DK009651, F32DK09651] Funding Source: Medline
Mutagenesis of Vibrio cholerae with TnphoA, followed by screening for fusions that were activated under low-iron conditions, led to the identification of seven independent fusion strains, each of which was deficient in the ability to utilize ferrichrome as a sole iron source for growth in a plate bioassay and had an insertion in genes encoding products homologous to Escherichia coli FhuA or FhuD. Expression of the gene fusions was independent of IrgB but regulated by Fur. We report here a map of the operon and the predicted amino acid sequence of FhuA, based on the nucleotide sequence. Unlike those of the E. coli fhu operon, the V. cholerac ferrichrome utilization genes are located adjacent and opposite in orientation to a gene encoding an ATP-binding cassette transporter homolog, but this gene, if disrupted, does not affect the utilization of ferrichrome in vitro.
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