期刊
BIOMATERIALS
卷 29, 期 7, 页码 894-903出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2007.10.055
关键词
silk; fibroin; stent; heparin; paclitaxel; clopidogrel
资金
- NHLBI NIH HHS [HL049973, R01 HL049973] Funding Source: Medline
- NIBIB NIH HHS [R01 EB003210, EB003210, P41 EB002520-03, P41 EB002520, R01 EB003210-04, EB002520] Funding Source: Medline
A multilayered silk fibroin protein coating system was employed as a drug carrier and delivery system to evaluate vascular cell responses to heparin, paclitaxel, and clopidogrel. The results demonstrated that the silk coating system was an effective system for drug-eluting coatings. such as for stent applications, based on its useful micromechanical properties and biological outcomes. Cell attachment and viability studies with human aortic endothelial cells (HAECs) and human coronary artery smooth muscle cells (HCASMCs) on the drug-incorporated silk coatings demonstrated that paclitaxel and clopidogrel inhibited smooth muscle cell (SMC) proliferation and retarded endothelial cell proliferation. Heparin-loaded silk multilayers promoted HAEC proliferation while inhibiting HCASMC proliferation, desired outcomes for the prevention of restenosis. The preservation of the phenotype of endothelial cells on silk and heparin-loaded silk coatings was confirmed with the presence of endothelial markers CD-31, CD-146, vWF and VE-Cadherin using immunocytochemistry assays. A preliminary in-vivo study in a porcine aorta showed integrity of the silk coatings after implantation and the reduction of platelet adhesion on the heparin-loaded silk coatings. (c) 2007 Elsevier Ltd. All rights reserved.
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