期刊
BIOMATERIALS
卷 29, 期 29, 页码 4003-4011出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2008.06.031
关键词
Fe3O4-Au; core-shell nanocrystals; streptavidin; magnetic activated cell separation; fluorescence-activated cell sorting
资金
- Korea Science and Engineering Foundation
- Ministry of Education, Science and Technology
- Korea Health 21 RD Project [A050750]
- Seoul RBD Program [10920]
- Korea Foundation for International Cooperation of Science and Technology [K20704000007-07A0500-00710, K20601000002-07E0100-00240]
- Innovative Research Institute for Cell Therapy [A062260]
- National Nuclear RD
- Korea Health Promotion Institute [A062260] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Ministry of Education, Science & Technology (MoST), Republic of Korea [2007-00476] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2007-00107] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We explored the feasibility of magnetite (Fe3O4)-gold (Au) core-shell nanocrystals as a useful vehicle for biomedical application such as cell separation. Streptavidin-fluorescein isothiocyanate (STA-FITC) was conjugated to the surface of the Fe3O4-Au core-shell nanocrystals using a carbodimide activation protocol. These nanocrystals were further tested for their ability to bind CD4(+) T lymphocytes, bound to biotin-labeled anti-CD4 mAbs, isolated from the spleen of C57BL/6 mice. Our data show that the Fe3O4-Au nanocrystals successfully pulled down CD4(+) T lymphocytes from the whole splenocytes with high specificity. Therefore, our nanocrystals provide an efficient tool for the cell separation process and further present the dramatic potential to be applied to other areas of biomedical application including diagnosis, monitoring, and treatment of human diseases. (C) 2008 Elsevier Ltd. All rights reserved.
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