期刊
BIOMATERIALS
卷 29, 期 17, 页码 2646-2655出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2008.02.019
关键词
cell stretching; polydimethylsiloxane; cell morphology; endothelial cell; epithelial cell; lung
资金
- NHLBI NIH HHS [R01 HL084370, R01 HL084370-03, HL084370-01] Funding Source: Medline
Cell culture systems are often static and are therefore nonphysiological. In vivo, many cells are exposed to dynamic surroundings that stimulate cellular responses in a process known as mechanotransduction. To recreate this environment, stretchable cell culture substrate systems have been developed, however, these systems are limited by being macroscopic and low throughput. We have developed a device consisting of 24 miniature cell stretching chambers with flexible bottom membranes that are deformed using the computer-controlled, piezoelectrically actuated pins of a Braille display. We have also developed efficient image capture and analysis protocols to quantify morphological responses of the cells to applied strain. Human dermal microvascular endothelial cells (HDMECs) were found to show increasing degrees of alignment and elongation perpendicular to the radial strain in response to cyclic stretch at increasing frequencies of 0.2, 1, and 5 Hz, after 2, 4, and 12 h. Mouse myogenic C2C12 cells were also found to align in response to the stretch, while A549 human lung adenocarcinoma epithelial cells did not respond to stretch. (c) 2008 Elsevier Ltd. All rights reserved.
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