期刊
BIOMASS & BIOENERGY
卷 64, 期 -, 页码 62-69出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biombioe.2014.03.027
关键词
Fermentation; Biomass; Cellulose; Consolidated bioprocessing; Ethanol; Hydrogen
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC) through the Hydrogen Canada (H2CAN) Network [138130-2011]
- Genome Canada through the Applied Genomics Research in Bioproducts or Crops (ABC)
- government of the Province of Manitoba through the Manitoba Research Innovation Fund (MRIF)
We have investigated a one-step fermentation process using purified hemp cellulose (PHC), hemp fibres (HF), and hemp hurds (HH), compared to reagent-grade alpha-cellullose (AC) for ethanol and hydrogen production by Clostridium thermocellum. Exponential phase growth and production rates on PHC were comparable to those observed with AC. Net production of ethanol for AC (8.47 mM) was only slightly higher than PHC (6.56 mM), but significantly higher than HF (5.48 mM) and HH (3.52 mM), while the final hydrogen yield was comparable for AC (12.70 mM), PHC (11.01 mM), HF (10.91 mM), and HH (4.72 mM). End-product yields were dependent on the intrinsic cellulosic content as well as the presence of other polymers present in substrate biomass. Rates of ethanol and hydrogen production were similar in early log phase but varied in mid-log, and accessibility to cellulose was shown to determine yield and metabolic flux distribution. Our data suggest that improving the accessibility of the cellulolytic bacterium, C. thermocellum, to cellulose fibres has a greater effect on increasing the yield of fermentation products than simply increasing the concentration of cellulose in the substrate. (C) 2014 Elsevier Ltd. All rights reserved.
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