期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 66, 期 4, 页码 1680-1684出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.66.4.1680-1684.2000
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Recombinant sucrose-6-phosphate synthase (SpsA) was synthesized in Escherichia coli BL21DE3 by using the spsA gene of the cyanobacterium Synechocystis sp. strain PCC 6803. Transformants exhibited a 10,000-fold increase in survival compared to wild-type cells following either freeze-drying, air drying, or desiccation over phosphorus pentoside, The phase transition temperatures and vibration frequencies (P=O stretch) in phospholipids suggested that sucrose maintained membrane fluidity during cell dehydration.
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