期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 40, 期 2, 页码 147-151出版社
ELSEVIER
DOI: 10.1016/S0167-7012(00)00118-4
关键词
cold-smoked fish; Listeria monocytogenes; rapid immunological-based methods
This study was conducted to evaluate the efficacy of the mini-VIDAS Listeria rnonocytogenes (LMO) system (BioMerieux Vitek, Inc., Missouri, USA) for detection of L.. monocytogenes in environmental and fish samples from three Portuguese cold-smoking plants and from their fresh fish suppliers. Mini-VIDAS-LMO is a fully automated system that uses fluorescent ELFA (Enzyme Linked Fluorescent Assay) technology for detection of Listeria monocytogenes antigens in food. It can be a rapid screening method alternative to time consuming classical isolation and identification. Two hundred and ninety five samples were tested in mini-VIDAS-LMO and in parallel by the ISO 11290-1 traditional protocol. The mini-VIDAS-LMO detected 8 of the 11 confirmed positive samples and presented 11 false positive results. The specificity of the mini-VIDAS-LMO found in this experiment was 0.96 and the sensitivity 0.73. (C) 2000 Elsevier Science B.V. All rights reserved.
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