期刊
ANALYTICA CHIMICA ACTA
卷 882, 期 -, 页码 68-75出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2015.04.012
关键词
Hypochlorous acid; Fluorescent probe; Boron dipyrromethene; Bioimaging
资金
- Ministry of Science and Technology (Taiwan) [MOST 103-2113-M-009-005]
- National Chiao Tung University
A BODIPY-based fluorescent probe, HBP, was developed for the detection of hypochlorous acid based on the specific hypochlorous acid-promoted oxidative intramolecular cyclization of heterocyclic hydrazone in response to the amount of HOCl. The reaction is accompanied by a 41-fold increase in the fluorescent quantum yield (from 0.004 to 0.164). The fluorescence intensity of the reaction between HOCl and HBP is linear in the HOCl concentration range of 1-8 mu M with a detection limit of 2.4 nM (S/N = 3). Confocal fluorescence microscopy imaging using RAW264.7 cells showed that the new probe HBP could be used as an effective fluorescent probe for detecting HOCl in living cells. (C) 2015 Elsevier B.V. All rights reserved.
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