期刊
BIOMASS & BIOENERGY
卷 35, 期 5, 页码 1878-1884出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biombioe.2011.01.031
关键词
Corncob; Cellulosic hydrolysates; Streptomyces sp.; Reducing sugar; Bioethanol
资金
- National Science Council of the Republic of China (Taiwan) [NSC 97-2313-B-264- 001-MY3]
Production of reducing sugar by hydrolysis of corncob material with Streptomyces sp. cellulase and ethanol fermentation of cellulosic hydrolysate was investigated. Cultures of Streptomyces sp. T3-1 improved reducing sugar yields with the production of CMCase, Avicelase and beta-glucosidase activity of 3.8, 3.9 and 3.8 IU/ml, respectively. CMCase, Avicelase, and beta-glucosidase produced by the Streptomyces sp. T3-1 favored the conversion of cellulose to glucose. It was recognized that the synergistic interaction of endoglucanase, exoglucanase and beta-glucosidase resulted in efficient hydrolysis of cellulosic substrate. After 5 d of incubation, the overall reducing sugar yield reached 53.1 g/100 g dried substrate. Further fermentation of cellulosic hydrolysate containing 40.5 g/l glucose was performed using Saccharomyces cerevisiae BCRC 21812, 14.6 g/l biomass and 24.6 g/l ethanol was obtained within 3 d. The results have significant implications and future applications regarding to production of fuel ethanol from agricultural cellulosic waste. (C) 2011 Elsevier Ltd. All rights reserved.
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