期刊
ANESTHESIOLOGY
卷 92, 期 4, 页码 1067-1073出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00000542-200004000-00025
关键词
depolarization; GABA(A) antagonist; mechanisms of anesthesia; neurotransmitter release; rat cerebrocortical slices
Background Many anesthetic agents are known to enhance the alpha(1)beta(2)gamma(2s) gamma-aminobutyric acid type A (GABA(A)) chloride current; however, they also depress excitatory neurotransmission. The authors evaluated two hypotheses: intravenous anesthetic agents inhibit glutamate release and any observed inhibition may be secondary to GABA, receptor activation. Methods: Cerebrocortical slices were prepared front Wistar rats. After perfusion in oxygenated Krebs buffer for 60 min at 37 degrees C, samples for glutamate assay were obtained at 2-min intervals, After 6 min, a 2-min pulse of 46 mM K+ was applied to the slices (S-1); this was repeated after 30 min (S-2). Bicuculline (1-100 mu M) was applied when the S, response returned to basal level, and 10 min later, thiopental (1-300 mu M), propofol(10 mu M), or ketamine (30 mu M) were also applied until the end of S-2. Perfusate glutamate concentrations were measured fluorometrically, and the area under the glutamate release curves was expressed as a ratio (S-2/S-1), Results: Potassium (46 mM) evoked a monophasic release of glutamate during S-1 and S-2, with a mean control S-2/S-1 ratio of 1.07 +/- 0.33 (mean +/- SD, n = 96). Ketamine and thiopental produced a concentration-dependent inhibition of K+-evoked glutamate release with half-maximum inhibition of release values of 18.2 and 10.9 mu M, respectively. Release was also inhibited by propofol, Bicuculline produced a concentration dependent reversal of thiopental Inhibition of glutamate release with a half-maximum reversal of the agonist effect of 10.3 mu M Bicuculline also reversed the effects of propofol but not those of ketamine, Conclusions: The authors' data indicate that thiopental, propofol, and ketamine inhibit K+-evoked glutamate release from rat cerebrocortical slices. The inhibition produced by thiopental and propofol is mediated by activation of GABA(A) receptors, revealing a subtle interplay between GABA-releasing (GABAergic) and glutamatergic transmission in anesthetic action.
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