期刊
IMMUNOLOGY LETTERS
卷 72, 期 1, 页码 7-15出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0165-2478(00)00162-0
关键词
phosphatidylinositol metabolism; signal transduction; B cell; Fc receptor
类别
Fc gamma RIIB are single-chain low-affinity receptors for IgG that bear an immunoreceptor tyrosine-based inhibition motif (ITIM) in their intracytoplasmic domain and that negatively regulate immunoreceptor tyrosine-based activation motif (ITAM)-dependent cell activation. In B cells, coaggregation of the B cell receptor (BCR) and Fc gamma RIIB leads to an inhibition of B cell activation. Inhibitory properties of Fc gamma RIIB have been related to the recruitment of SHIP, an SH2 domain-containing inositol 5-phosphatase (referred to as SHIP1), via ITIM phosphorylated Fc gamma RIIB. Here, we demonstrate that the second SH2 domain-containing inositol 5-phosphatase SHIP2 could also bind to the Fc gamma RIIB ITIM. As a model, a Fc gamma RIIB deficient B cell line (IIA1.6), transfected with a cDNA encoding either w.t. Fc gamma RIIB1' or Fc gamma RIIB1' whose ITIM tyrosine was mutated has been used. SHIP2 tyrosine phosphorylation and association to the adaptator protein Shc were only found in transfectants expressing w.t. Fc gamma RIIB1'. SHIP2 was also found to bind to a phosphopeptide corresponding to the ITIM sequence of Fc gamma RIIB. There was no binding to the nonphosphorylated peptide. Finally, both SHIP2 and SHIP1 were coprecipitated with Fc gamma RIIB1' upon coaggregation with BCR in IIA1.6 transfectants. (C) 2000 Elsevier Science B.V. All rights reserved.
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