A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae

Gene tagging can be achieved by homologous recombination in yeast. The kan(t) marker gene plays an important role in PCR-mediated gene disruption and PCR-mediated epitope tagging experiments. In this paper, new modules containing a tag-loxP-kanMX-loxP cassette are described that allow tagging of different genes by using the kan(r) marker repeatedly. (C) 2000 Elsevier Science B.V. All rights reserved.