期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 55, 期 1-3, 页码 193-200出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0168-1605(00)00163-X
关键词
viability; fluorescence techniques; membrane integrity; respiration; intracellular pH; microorganisms
Viability assessment of microorganisms is relevant for a wide variety of applications in industry, including evaluation of inactivation treatments and quality assessment of starter cultures for beer, wine, and yoghurt production. Usually, the ability of microbial cells to reproduce is considered as the benchmark method for determination of cell viability, and this is most commonly determined by the plate count method. The time needed to form visible colonies, however, is relatively long. Therefore, there is an increasing interest in rapid methods which exploit criteria other than reproduction. In this review the applications of fluorescent probes for, e.g., determination of membrane integrity, enzyme activities, respiration, membrane potential and intracellular pH, are discussed in detail. (C) 2000 Elsevier Science BSI. All rights reserved.
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