Sensitive determination of 17β-estradiol in river water using a graphene based electrochemical sensor

In this study, a novel material for the electrochemical determination of 17 beta-estradiol using an electrode based on reduced graphene oxide and a metal complex porphyrin has been applied to environmental monitoring. The electrochemical profile of the proposed electrode was analyzed by differential pulse voltammetry, which showed a shift of the oxidation peak potential of 17 beta-estradiol to 150 mV in a less positive direction compared to the bare reduced graphene oxide electrode. DPV experiments were performed in PBS at pH 7.0 to determine 17 beta-estradiol without any previous step of extraction, cleanup, or derivatization, in the range of 0.1-1.0 mmol L-1 with a detection limit archived at 5.3 nmol L-1 ( 1.4 mg L-1). The proposed sensor was successfully applied in the determination of 17 beta-estradiol in a river water sample without any purification step and was successfully analyzed under the standard addition method. All the obtained results were in agreement with those from the HPLC procedure. (C) 2015 Elsevier B.V. All rights reserved.