Genetic and cellular defects contributing to benign tumor formation in neurofibromatosis type 1

Neurofibromatosis type 1 (NF1) is a common inherited cancer predisposition syndrome, The NF1 gene product, neurofibromin, is hypothesized to function as a tumor suppressor and nearly all NF1 patients develop benign peripheral nerve tumors, These neurofibromas presumably arise from NF1 inactivation in S100(+) Schwann cells, but there is no formal proof for this mechanism. We demonstrate that fibroblasts isolated from neurofibromas carried at least one normal NF1 allele and expressed both NF1 mRNA and protein, whereas the S100(+) cells typically lacked the NF1 transcript. Our findings further indicate that additional molecular events aside from NF1 inactivation in Schwann cells and/or other neural crest derivatives contribute to neurofibroma formation.