期刊
BIOMARKERS IN MEDICINE
卷 2, 期 6, 页码 613-616出版社
FUTURE MEDICINE LTD
DOI: 10.2217/17520363.2.6.613
关键词
biomarker; podocyte; urinary podocytes; urinary podocyte-specific molecules
Podocyturia may be an expression of glomerular disease and is evaluated using urinary podocyte-specific molecules. This article discusses the technical problems of detection and quantification of podocyturia using immunofluorescent staining of specific proteins, urinary podocyte culture, flow cytometry and urinary podocyte-specific molecules (including PCR, immunoblotting and ELISA). Technical problems mean that it is still impossible to define a biomarker for urinary podocyte injury. This article also discusses the future perspectives for podocyturia studies, including fluorescence-activated cell sorting and the study of the proteome of urinary exosomes.
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