期刊
CARBOHYDRATE RESEARCH
卷 325, 期 3, 页码 202-210出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0008-6215(99)00309-2
关键词
transgalactosylation; beta-D-galactopyranosyl-(1 -> 3)-beta-D-galactopyranosyl-(1 -> 4)-D-glucose; 3 '-beta-galactopyranosyllactose
Enzymatic transgalactosylation of lactose by means of Streptococcus thermophilus, subspecies DN-001065, led to a mixture of D-galactose (similar to 4%), D-glucose ( similar to 15%), lactose (similar to 51%), minor disaccharides (6%), trisaccharides ( similar to 20%) and tetrasaccharides (3%). The major trisaccharide ( similar to 16%) was identified by NMR spectroscopy and chemical synthesis as bring the known beta-D-galactopyranosyl-(1-->3)-beta-D-galactopyranosyl-(1-->4)-D-glucose (3'-beta-D-galactopyranosyl-lactose). It was purified from a mixture of peracetylated oligosaccharides by column chromatography followed by deacetylation. For the first time, 3'-beta-D-galactopyranosyl-lactose has been obtained on the 1 g scale, by resorting to simple techniques and equipment. NMR spectra have been unambiguously assigned. (C) 3000 Elsevier Science Ltd. All rights reserved.
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