期刊
FEBS LETTERS
卷 472, 期 1, 页码 39-44出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)01425-3
关键词
PC-12 cell; anandamide; superoxide; CPP32-like protease; apoptosis
Anandamide (arachidonoylethanolamide), an endogenous cannabinoid receptor ligand has been suggested to have physiological role in mammalian nervous system. However, little is known about the role of anandamide on neuronal cells, Here, we demonstrate that anandamide causes death of PC-12 cells, shoeing marked DNA condensation and fragmentation, appearance of cells at sub-G(0)/G(1) and redistribution of phosphatidyl serine, the hallmark features of apoptosis, Anandamide raised intracellular superoxide level and CPP32-like protease activity in PC-12 cells markedly. Furthermore, antioxidant N-acetyl cysteine prevented anandamide-induced superoxide anion formation and cell death, implying that intracellular superoxide is a novel mediator of anandamide-induced apoptosis of PC-12 cells, (C) 2000 Federation of European Biochemical Societies.
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