期刊
TALANTA
卷 51, 期 5, 页码 835-847出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0039-9140(99)00335-5
关键词
cell sorting; methodology; field flow fractionation
Field flow fractionation (FFF) methods were conceptualised in the late 1960s by J.C Giddings. These techniques are particularly suited for the retention and separation of micron and sub-micron sized particles. Systematic technological development as well as methodological procedures were established to achieve separations over the last 30 years. The elution mechanism of micron sized species is now known as 'steric/hyperlayer'. Cells are micron sized particles of life science interest, in particular those living in suspension. The separation of cells according to differences in their biophysical characteristics is therefore possible using the FFF principle. In the first part of this report, characteristics of classical cell separation methodologies are recounted as well as the specific features of FFF. In the second part, a review of cell separations or purifications obtained with sedimentation FFF techniques is given and FFF trends in cell separation is developed. (C) 2000 Elsevier Science B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据