期刊
BIOMACROMOLECULES
卷 15, 期 4, 页码 1526-1533出版社
AMER CHEMICAL SOC
DOI: 10.1021/bm500199h
关键词
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资金
- Fonds der Chemischen Industrie (FCI)
- Max Planck Graduate Center
- Johannes Gutenberg-Universitat Mainz (MPGC)
- DFG Sonderforschungsbereich [SFB 1066]
- Grants-in-Aid for Scientific Research [26670738, 23790043] Funding Source: KAKEN
For systemic siRNA delivery applications, well-defined drug carriers are required that guarantee stability for both carrier and cargo. Among various concepts progressing in market or final development, cationic nanohydrogel particles may serve as novel transport media especially designed for siRNA-in vivo experiments. In this work, the interaction of nanohydrogel particles with proteins and serum components was studied via dynamic light scattering in human blood serum as novel screening method prior to applications in vivo. The formation of larger aggregates mostly caused by charge interaction with albumin could be suppressed by nanogel loading with siRNA affording a neutral zeta potential for the complex. Preliminary in vivo studies confirmed the results inside the light-scattering cuvette. Although both carrier and cargo may have limited stability on their own under physiological relevant conditions, they can form safe and stable complexes at a charge neutralized ratio and thus making them applicable to systemic siRNA delivery.
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