期刊
BIOMACROMOLECULES
卷 15, 期 4, 页码 1185-1193出版社
AMER CHEMICAL SOC
DOI: 10.1021/bm401740x
关键词
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资金
- National High Technology Research and Development Program 863 [2013AA102507]
- Zhejiang Provincial Natural Science Foundation of China [LZ12C17001]
- National Natural Science Foundation of China [20804037, 21172194]
- Silkworm Industry Science and Technology Innovation Team [2011R50028]
- Projects of Zhejiang Provincial Science and Technology Plans [2012C12910]
- National Institutes of Health [EB015190, HL092526]
- National Science Foundation [CBET-0854465, CMMI-1234957, CBET-0854414, DMR-0847758]
- Department of Defense Peer Reviewed Medical Research Program [W81XWH-12-1-0384]
- Oklahoma Center for Adult Stem Cell Research [434003]
- Oklahoma Center for the Advancement of Science and Technology [HR11-006]
- Directorate For Engineering
- Div Of Civil, Mechanical, & Manufact Inn [1234957] Funding Source: National Science Foundation
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [847758] Funding Source: National Science Foundation
Biomacromolecules have been used as templates to grow hydroxyapatite crystals (HAps) by biomineralization to fabricate mineralized materials for potential application in bone tissue engineering. Silk sericin is a protein with features desirable as a biomaterial, such as increased hydrophilicity and biodegradation. Mineralization of the silk sericin from Antheraea pernyi (A. pernyi) silkworm has rarely been reported. Here, for the first time, nucleation of HAps on A. pernyi silk sericin (AS) was attempted through a wet precipitation method and consequently the cell viability and osteogenic differentiation of BMSCs on mineralized AS were investigated. It was found that AS mediated the nucleation of HAps in the form of nanoneedles while self-assembling into beta-sheet conformation, leading to the formation of a biomineralized protein based biomaterial. The cell viability assay of BMSCs showed that the mineralization of AS stimulated cell adhesion and proliferation, showing that the resultant AS biomaterial is biocompatible. The differentiation assay confirmed that the mineralized AS significantly promoted the osteogenic differentiation of BMSCs when compared to nonmineralized AS as well as other types of sericin (B. mori sericin), suggesting that the resultant mineralized AS biomaterial has potential in promoting bone formation. This result represented the first work proving the osteogenic differentiation of BMSCs directed by silk sericin. Therefore, the biomineralization of A. pernyi silk sericin coupled with seeding BMSCs on the resultant mineralized biomaterials is a useful strategy to develop the potential application of this unexplored silk sericin in the field of bone tissue engineering. This study lays the foundation for the use of A. pernyi silk sericin as a potential scaffold for tissue engineering.
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