期刊
BIOMACROMOLECULES
卷 15, 期 9, 页码 3278-3289出版社
AMER CHEMICAL SOC
DOI: 10.1021/bm500670r
关键词
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资金
- Singapore Ministry of Education Tier 2 Grant [MOE 2011-T2-2-044]
- Singapore National Research Foundation (NRF) through a NRF Fellowship
- Biomedical Research Council (BMRC) of the Agency for Science, Technology, and Research (A*STAR) of Singapore
The sucker ring teeth (SRT) of Humboldt squid exhibit mechanical properties that rival those of robust engineered synthetic polymers. Remarkably, these properties are achieved without a mineral phase or covalent cross-links. Instead, SRT are exclusively made of silk-like proteins called suckerins, which assemble into nanoconfined beta-sheet reinforced suprarnolecular networks. In this study, three streamlined strategies for full-length recombinant suckerin protein production and purification were developed. Recombinant suckerin exhibited high solubility and colloidal stability in aqueous-based solvents. In addition, the colloidal suspensions exhibited a concentration-dependent conformational switch, from random coil to beta-sheet enriched structures. Our results demonstrate that recombinant suckerin can be produced in a facile manner in E. coli and processed from mild aqueous solutions into materials enriched in beta-sheets. We suggest that recombinant suckerin-based materials offer potential for a range of biomedical and engineering applications.
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