期刊
BIOMACROMOLECULES
卷 15, 期 3, 页码 1062-1068出版社
AMER CHEMICAL SOC
DOI: 10.1021/bm500062n
关键词
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资金
- National High Technology Research and Development Program (863 Program) of China [2012AA020501]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA01030000, XDA04020000]
- National Natural Science Foundation of China [31200813]
- Ministry of Science and Technology of China [2011CB965001]
- Military Medical Project [BWS11J002]
Neural stem/progenitor cells (NS/PCs) play a therapeutic role in nervous system diseases and contribute to functional recovery. However, their efficacy is limited as the majority of cells die post-transplantation. In this study, collagen sponges were utilized as carriers for NS/PCs. Basic fibroblast growth factor (bFGF), a mitogen for NS/PCs, was incorporated into the collagen sponges to stimulate NS/PC proliferation. However, the effect of native bFGF is limited because it diffuses into the culture medium and is lost following medium exchange. To overcome this problem, a collagen-binding polypeptide domain, which has high affinity to collagen, was fused with bFGF to sustain the exposure of NS/PCs within the collagen sponges to bFGF. The results indicated that the number of NS/PCs was significantly higher in collagen sponges incorporating engineered bFGF than in those with native bFGF or the PBS control after 7 days in culture. Here, we designed a natural biological neural scaffold consisting of collagen sponges, engineered bFGF, and NS/PCs. In addition to the effect of proliferated NS/PCs, the engineered bFGF retained in the natural biological neural scaffolds could have a direct effect on nervous system reconstruction. The two aspects of the natural biological neural scaffolds may produce synergistic effects, and so they represent a promising candidate for nervous system repair.
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