期刊
ANALYTICA CHIMICA ACTA
卷 891, 期 -, 页码 291-297出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2015.08.012
关键词
Fluorescent probe; Molecular beacon; Gold nanoparticles; Aptamer; Organophosphorus pesticides
资金
- National Natural Science Foundation of China [81274072, 81473346]
- Specialized Research Fund for Major Science and Technology Program of Hainan, China [ZDZX2013008]
- PUMC Youth Fund
- Fundamental Research Funds for the Central Universities [33320140146]
- Xiehe New Star Project
A nanomaterials-based novel molecular beacon has attracted growing attentions in fluorescent assays as many nanomaterials possess excellent quenching efficiency. In this work, a gold-based nanobeacon probe was established to detect organophosphorus pesticides for the first time. The constructed gold-based nanobeacon acted as a signal indicator and could display the decreasing of the intensity in the presence of targets, which competitively bound to single strand DNA. To achieve a high sensitive probe, some parameters including solution pH, temperature and reaction time were investigated and optimized. The gold-based nanobeacon probe assay was proved to be rapid and sensitive to achieve a detection limit of 0.035 mu M for isocarbophos, 0.134 mu M for profenofos, 0.384 mu M for phorate and 2.35 mu M for omethoate, respectively. The prepared nanobeacon effectively reduced the background and improved the detection sensitivity and selectivity. The probe is stable, easy to operate and does not need sophisticated instruments. These features makes the probe feasible for screening trace organophosphorus pesticides in real samples. (C) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据