期刊
JOURNAL OF CELL BIOLOGY
卷 149, 期 3, 页码 647-656出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.149.3.647
关键词
cell polarity; cytoskeleton; Drosophila melanogaster; plasma membrane; ankyrins
类别
资金
- NIDDK NIH HHS [DK42086] Funding Source: Medline
- NIGMS NIH HHS [GM49301] Funding Source: Medline
Spectrin has been proposed to function as a sorting machine that concentrates interacting proteins such as the Na,K ATPase within specialized plasma membrane domains of polarized cells. However, little direct evidence to support this model has been obtained. Here we used a genetic approach to directly test the requirement for the beta subunit of the alpha beta spectrin molecule in morphogenesis and function of epithelial cells in Drosophila. beta Spectrin mutations were lethal during late embryonic/early larval development and they produced subtle defects in midgut morphology and stomach acid secretion. The polarized distributions of alpha beta(H) spectrin and ankyrin were not significantly altered in beta spectrin mutants, indicating that the two isoforms of Drosophila spectrin assemble independently of one another, and that ankyrin is upstream of alpha beta spectrin in the spectrin assembly pathway. In contrast, beta spectrin mutations had a striking effect on the basolateral accumulation of the Na,K ATPase. The results establish a role for beta spectrin in determining the subcellular distribution of the Na,K ATPase and, unexpectedly, this role is independent of alpha spectrin.
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