期刊
BIOMACROMOLECULES
卷 13, 期 7, 页码 2099-2109出版社
AMER CHEMICAL SOC
DOI: 10.1021/bm3004836
关键词
-
资金
- Department of Energy [DE-SC0005166]
- Baystate Health Systems (Springfield, MA)
- National Science Foundation [CBET-0932781]
- National Science Foundation through a Graduate Research Fellowship
- [06-4]
Novel pentafluorophenyl (PFP)-ester-functionalized phosphorylcholine (PC) polymers of different architectures were prepared and conjugated to lysozyme as a model protein. Linear and two-arm poly(2-methacryloyloxyethyl phosphorylcholine) (polyMPC) structures containing PFP functionality at the chain-end were prepared by atom transfer radical polymerization (ATRP) from novel initiators. Additional conjugates were prepared from phosphorylcholine-substituted cyclooctene (PC-COE) polymers containing PFP-ester bearing comonomers. The polymer-protein conjugates were characterized by HPLC, FPLC, and DLS and were seen to retain most (similar to 80% or greater) of their native enzymatic activity. Pharmacokinetic profiles of the polymer-protein conjugates were studied in mice and found to increase the circulation half-life compared with lysozyme alone.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据