期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 40, 期 3, 页码 233-239出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0167-7012(00)00126-3
关键词
biofilm; anaerobic; laser confocal microscopy; Porphyromonas gingivalis; plaque; periodontal disease
We have developed an anaerobic biofilm culture system. The system is inexpensive, simple to use and, unlike an anaerobic glovebox, requires no dedicated space. As a test of the system, Porphyromonas gingivalis was cultured under low oxygen (1-2 ppm) and under anaerobic conditions (less than or equal to 0.1 ppm O-2). In the presence of small amounts of oxygen, the organism attached and formed an initial biofilm over the course of 4 h, but the biofilm was unable to maintain its growth and had lost biomass after Is h. Also, ambiguous results were obtained when the biofilm was stained with a viability stain. Under anaerobic conditions, the biofilm was able to continue growth - biomass was greater after 18 h than after 4 h, and the anaerobic biofilm had a less ambiguous staining pattern than did the low-O-2-grown biofilm. (C) 2000 Published by Elsevier Science B.V. All rights reserved.
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