期刊
BIOMACROMOLECULES
卷 12, 期 6, 页码 2193-2199出版社
AMER CHEMICAL SOC
DOI: 10.1021/bm200274r
关键词
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资金
- National Science Foundation [CBET-0854414, DMR-0847758, CBET-0854465]
- National Institutes of Health [R01 HL092526-01A2, R03AR056848-01, R21EB009909-01A1, DE11657]
- Oklahoma Center for the Advancement of Science and Technology [HR06-161S]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [847758] Funding Source: National Science Foundation
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0854465, 0854414] Funding Source: National Science Foundation
BioinsPired mineralization is an innovative approach to the fabrication of bone biomaterials mimicking the natural bone. Bone mineral hydroxylapatite (HAP) is preferentially oriented with c-axis parallel to collagen fibers in natural bone. However, such orientation control is not easy to achieve in artificial bone biomaterials. To overcome the lack of such orientation control, we fabricated a phage-HAP composite by genetically engineering M13 phage, a nontoxic bionanofiber, with two HAP-nucleating peptides derived from one of the noncollagenous proteins, Dentin Matrix Protein-1 (DMP1). The phage is a biological nanofiber that can be mass produced by infecting bacteria and is nontoxic to human beings. The resultant HAP-nucleating phages are able to self-assemble into bundles by forming beta-structure between the peptides displayed on their side walls. The beta-structure further promotes the oriented nucleation and growth of HAP crystals within the nanofibrous. phage bundles with their c-axis preferentially parallel to the bundles. We proposed that the preferred orientation resulted from the stereochemical matching negatively acid residues withinn the beta-structure and the positively charged calcium ions on the (001) plane of HAP crystals. The self-assembly and minerlization driven by the beta-structure formation represent a new route for fabricating mineralized fibers that can serve as building blocks in forming bone repair biomaterials and mimic the basic structure of natural bones
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