期刊
BIOMACROMOLECULES
卷 12, 期 11, 页码 3945-3951出版社
AMER CHEMICAL SOC
DOI: 10.1021/bm200962s
关键词
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资金
- Japan Science and Technology Agency (JST)
- New Energy and Industrial Technology Development Organization (NEDO)
- Institut Universitaire de France (IUF)
- European Research Council (ERC) under the European Community [258782]
- Grants-in-Aid for Scientific Research [23651203, 23681042] Funding Source: KAKEN
AzoTAB, a photosensitive azobenzene cationic surfactant, which phototriggers translation activity through light-regulated condensation of mRNA, is added to a translation solution containing several mRNAs, which can be selectively silenced by specific small RNAs. We find that gene silencing by small RNAs remains functional regardless of AzoTAB concentration and UV illumination. In the absence of UV, the translation of all genes present in the medium is partially to fully inhibited depending on AzoTAB concentration. In contrast, the application of a short UV stimulus (365 nm for 1.5 min) results in the selective photoactivation of genes that are not silenced by small RNA. These results show that light-regulated condensation by AzoTAB works as a sequence-independent series photoswitch added to parallel sequence-specific regulation by small RNAs.
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