期刊
BIOMACROMOLECULES
卷 12, 期 2, 页码 482-493出版社
AMER CHEMICAL SOC
DOI: 10.1021/bm101272g
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资金
- NCCR Nanoscale Science
- Fonds Quebecois de la Recherche sur la Nature et les Technologies (Quebec, Canada)
In this study, the residue-selective modification of proteins with polymers at arginine residues is reported. The difficulty in modifying arginine residues lies in the fact that they are less reactive than lysine residues. Consequently, typical chemo-selective reactions which employ kinetic selectivity (active esters, Michael addition, etc.) cannot be used to target these residues. The chemistry exploited herein relies on thermodynamic selectivity to achieve selective modification of arginine residues. omega-Methoxy poly(ethylene glycol) bearing an alpha-oxo-aldehyde group was synthesized and used to demonstrate the selective modification of lysozyme at arginine residues. In addition, the optimization of reaction conditions for coupling as well as the stability of the formed adduct toward dilution, toward a nucleophilic buffer, and toward acidification are reported. It was concluded that this approach is a convenient, mild, selective, and catalyst-free method for protein modification.
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