期刊
BIOMACROMOLECULES
卷 11, 期 10, 页码 2583-2592出版社
AMER CHEMICAL SOC
DOI: 10.1021/bm100521x
关键词
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资金
- NIH [R01 HL64387]
- Coulter Foundation
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL064387] Funding Source: NIH RePORTER
We have developed a thermoresponsive poly(N-isopropyl acrylamide)-based scaffold with degradability and controlled porosity. Biodegradable poly(N-isopropyl acrylamide) hydrogels were synthesized by photocopolymerization of N-isopropylacrylamide with 2-methylene-1,3-dioxepane and polycaprolactone dimethacrylate. The hydrogels' phase transition temperature, swelling, and viscoelastic properties, as well as hydrolytic degradability at 25 and 37 degrees C, were explored. A sphere-templating technique was applied to fabricate hydrogel scaffolds with controllable pore size and a highly interconnected porous structure. The scaffold pore diameter change as a function of temperature was evaluated and, as expected, pores decreased in diameter when the temperature was raised to 37 degrees C. 3-(4,5-Dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) test results suggested neither the scaffolds nor their degradation products were cytotoxic to NIH3T3 cells. Scaffolds-with 55 +/- 5 mu m pore diameter were loaded with NIH3T3 cells and then were warmed to 37 degrees C entrapping cells in pores approxitnately 39 mu m in diameter, a size range we have found to be optimal for angiogenesis and biointegration. Cells showed uniform infiltration and an elongated morphology after 7 days of culture. Due to the controlled monodisperse pore diameter. highly interconnected architecture, fully degradable chemistry and thermoresponsive properties, the polyNIPAM-based scaffolds developed here are attractive for applications in tissue engineering.
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