期刊
BIOMACROMOLECULES
卷 11, 期 2, 页码 489-495出版社
AMER CHEMICAL SOC
DOI: 10.1021/bm901215s
关键词
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资金
- National Natural Science Foundation of China [50773037, 20774057, 50633010]
- National Basic Research Program [2009CB930400]
- Fok Ying Tung Education Foundation [111048]
- Shuguang Program [08SG14]
- Science and Technologgy Commission of Shanghai Municipality [0852 nm05800]
- Shanghai Leading Academic Discipline Project [B202]
A general strategy to improve the transfection efficiency as well as lower the cytotoxicity for polycationic vectors has been developed Through the polycondensation addition of N,N'-methylene bisacrylamide and 1-(2-aminoethyl)piperazine in a water/N,N-dimethylformamide cosolvent, a series of cationic poly(amido amine)s with same repeating units but different branched architecture have been prepared. With the Increase in branched architecture, the cationic polymers become more and more compact, accompanied by the enhancement of primary and tertiary amino groups. Therefore, the buffering capacities and DNA condensation capabilities of cationic poly(amido amine)s are strengthened greatly, whereas the correspondent cytotoxicity decreases. Correspondingly, the transfection efficiency is improved by more than three orders of magnitude. The results of this Study indicate that the gene delivery can be readily regulated by only changing the branched architecture of polycations.
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