期刊
BIOMACROMOLECULES
卷 11, 期 4, 页码 1036-1042出版社
AMER CHEMICAL SOC
DOI: 10.1021/bm1000108
关键词
-
资金
- Ministry of Education, Science, Sports and Culture [17680039]
- Iketani Science and Technology Foundation
- Grants-in-Aid for Scientific Research [17680039] Funding Source: KAKEN
To improve the stability of a head tail type polycation (PAMAM dendron-PLL), which is composed of a polyamidoamine (PAMAM) dendron head and poly(L-lysine) (PLL) tail blocks, as a gene vector against serum proteins, the PEGylation of the PAMAM dendron head was examined using physicochemical and transfection experiments. The PEGylation of the PAMAM dendron-PLL polyplexes neutralized the zeta-potential, indicating that the PEG brushes surround the surface of the polyplexes. The PEGylated PAMAM dendron-PLL polyplexes had higher tolerance against incubation with serum proteins when compared with non-PEGylated PAMAM dendron-PLL polyplexes. In addition, the transfection efficiency of the PEGylated PAMA:M dendron-PLL polyplexes increased with increasing incubation periods, leading to an increase in the amount of pDNA uptaked by He La cells. Furthermore, effective stabilization of the PAMAM dendron-PLL polyplexes through PEGylation of the PAMAM dendron block was confirmed by the transfection experiments after preincubation with serum proteins and the PEGylated PAMAM dendron-PLL polyplexes showed a comparable level of transfection efficiency with polyethyleneimine polyplexes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据