期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 66, 期 5, 页码 2216-2219出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.66.5.2216-2219.2000
关键词
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An immunofluorescent method involving double color labeling and confocal microscopy was reported to specifically detect lactic acid bacteria and probiotic cells coimmobilized in gels beads. The method described is rapid (4 h) and sensitive and may be useful for studying cell dynamics during mixed-culture starter production using immobilized cells in gel beads. Microscopic observations were perfectly correlated to cell counts obtained using a sandwich enzyme linked immunosorbent assay.
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