期刊
BIOLOGY OF REPRODUCTION
卷 91, 期 6, 页码 -出版社
OXFORD UNIV PRESS INC
DOI: 10.1095/biolreprod.114.122796
关键词
comparative genetics; DMRT1; FOXL2; goat; RNA sequencing; sex-determination; XX sex reversal
资金
- Agence Nationale pour la Recherche [ANR-09-GENM-009-03]
- Universite Paris Sud-11
- Fondation pour la Recherche Medicale [FDT20140930877]
- GENIDOV [ANR-09-GENM-009-03]
FOXL2 loss of function in goats leads to the early trans-differentiation of ovaries into testes, then to the full sex reversal of XX homozygous mutants. By contrast, Foxl2 loss of function in mice induces an arrest of follicle formation after birth, followed by complete female sterility. In order to understand the molecular role of FOXL2 during ovarian differentiation in the goat species, putative FOXL2 target genes were determined at the earliest stage of gonadal sex-specific differentiation by comparing the mRNA profiles of XX gonads expressing the FOXL2 protein or not. Of these 163 deregulated genes, around two-thirds corresponded to testicular genes that were up-regulated when FOXL2 was absent, and only 19 represented female-associated genes, down-regulated in the absence of FOXL2. FOXL2 should therefore be viewed as an antitestis gene rather than as a female-promoting gene. In particular, the key testis-determining gene DMRT1 was found to be up-regulated ahead of SOX9, thus suggesting in goats that SOX9 primary up-regulation may require DMRT1. Overall, our results equated to FOXL2 being an antitestis gene, allowing us to propose an alternative model for the sex-determination process in goats that differs slightly from that demonstrated in mice.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据