期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 10, 期 9, 页码 891-894出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0960-894X(00)00127-X
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资金
- NCI NIH HHS [CA 72925] Funding Source: Medline
A new panel of steroidal cationic lipids has been synthesized for gene delivery. Using commercially available vitamin D-2 (calciferol) or vitamin D-3 (cholecalciferol) as hydrophobic motifs and a variety of cationic head groups as binding sites for negatively charged phosphate groups in DNA, we demonstrated that the transfection activity of the synthetic vitamin D-based cationic lipids 1d, 2d formulated with dioleoylphosphatidylethanolamine (DOPE) as a co-lipid is comparable to that of 3-(-[N-N',N'-dimethylaminoethane)carbamoyl]cholesterol (DC-Chol). These synthetic lipids are effective in transfecting a variety of cell lines. These results suggest that vitamin D-based cationic lipids are useful transfection reagents for in vitro gene transfer studies. (C) 2000 Published by Elsevier Science Ltd.
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