The soil food web of two beech forests (Fagus sylvatica) of contrasting humus type:: stable isotope analysis of a macro- and a mesofauna-dominated community

The structure of the soil food web in two beech (Fagus sylvatica) forests, the Gottinger Wald and the Selling forest (Northern Germany), was investigated using variations in tissue N-15 concentrations of animal species or taxa. The Gottinger Wald is located on a limestone plateau and characterized by mull humus with high macrofauna activity, particularly of Lumbricidae, Diplopoda and Isopoda. In contrast, the Selling forest is located on a sandstone mountain range and characterized by moder humus. The soil fauna of this forest is dominated by mesofauna, particularly by Collembola, Enchytraeidae and Oribatida. In June 1995 soil fauna was sampled using heat extraction. Three soil layers were analysed at each of the sites. N-15/N-14 ratios of bulk material increased strongly with soil depth in both forests. This also applied to the water-soluble fraction at the Gottinger Wald, but not at the Selling. Generally, the water-soluble fraction was more enriched in N-15 than the bulk materials. For most animals studied N-15/N-14 ratios varied little with soil depth. In both forests soil animals could be classified either as saprophages, including microphytophages, or predators. On average, the delta(15)N of predatory taxa (Chilopoda, Araneida, Gamasina, Staphylinidae) exceeded that of saprophagous or microphytophagous taxa (Lumbricidae, Isopoda, Diplopoda, Collembola, Oribatida, Enchytraeidae) by 4.4 and 3.9 parts per thousand for the Gottinger Wald and the Selling, respectively. We assume that most of the saprophagous or microphytophagous taxa studied consist of primary and secondary decomposers and hypothesize that predators prey more on secondary than primary decomposers. Generally, average delta(15)N values differed little be-tween saprophagous (Lumbricidae, Diplopoda, Isopoda) and microphytophagous taxa (Collembola, Oribatida). The variations in delta(15)N values of species within these taxa consistently exceeded the variation between them, indicating that the species of each of these taxa form a continuum from primary to secondary decomposers. Also, variations in delta(15)N values within predatory taxa in most cases exceeded that between taxa excluding top predators like Sorer. We conclude that using higher taxonomic units in soil food web analysis is problematic and in general not consistent with nature. Higher taxonomic units may only be useful for depicting very general trophic groupings such as predators or microbi-detritivores.