期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 377, 期 1, 页码 43-48出版社
ACADEMIC PRESS INC
DOI: 10.1006/abbi.2000.1739
关键词
electrochemical reduction; bioelectrochemistry; cytochrome P4502B4; cytochrome P450 1A2; flavocytochrome 2B4; flavocytochrome 1A2; screen-printed thick-film electrodes; electrocatalytic reduction
The present study shows that cytochromes P450 2B4 and 1A2 with a covalently attached riboflavin (semisynthetic flavocytochromes RfP450 2B4 and RfP450 1A2) can be reduced electrochemically on rhodium-graphite electrodes at a potential of -500 mV (vs Ag/AgCl). In the presence of substrates such as aminopyrine, aniline, 7-ethoxyresorufin, and 7-pentoxyresorufin, N-demethylation, p-hydroxylation, and O-dealkylation reactions proceeded, as was confirmed by product analysis. Rates of electrocatalytically driven reactions are comparable to those obtained using NAD(P)H as the source of reducing equivalents. These results suggest the practicality of developing flavocytochrome P450s as catalysts for oxidation reactions with different classes of organic substrates. (C) 2000 Academic Press.
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