期刊
JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY
卷 21, 期 4, 页码 313-319出版社
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1005679215704
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- NIAMS NIH HHS [AR10551] Funding Source: Medline
The goal of this study was to characterize the interrelationship between sarcomere length and interfilament spacing in the control of Ca2+ sensitivity in skinned rabbit psoas muscle fibers. Measurements were made at sarcomere lengths 2.0, 2.7 and 3.4 mu m. At 2.7 mu m the fiber width was reduced by 17% relative to that at 2.0 mu m and the pCa(50) for force development was increased by similar to 0.3 pCa units. In the presence of 5% Dextran T-500 the fiber width at sarcomere length 2.0 mu m was also decreased by 17% and the Ca2+ sensitivity was increased to the same value as at 2.7 mu m. In contrast, at sarcomere length 2.7 mu m the addition of as much as 10% Dextran T-500 had no effect on Ca2+ sensitivity. At sarcomere length 3.4 mu m there was an additional 7% compression and the Ca2+ sensitivity was increased slightly (similar to 0.1 pCa units) relative to that at 2.7 mu m. However at 3.4 mu m the addition of 5% Dextran T-500 caused the Ca2+ sensitivity to decrease to the level seen at 2.0 mu m. Given that the skinning process causes a swelling of the filament lattice it is evident that the relationship between sarcomere length and Ca2+ sensitivity observed in skinned fibers may not always be applicable to intact fibers. These data are consistent with measurements of Ca2+ in intact fibers which indicate that there might be a decline in Ca2+ sensitivity at long sarcomere lengths.
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