期刊
BIOLOGY OF REPRODUCTION
卷 84, 期 1, 页码 97-105出版社
OXFORD UNIV PRESS INC
DOI: 10.1095/biolreprod.110.086462
关键词
culture; developmental biology; gametogenesis self renewal; Sertoli cells; serum; spermatogenesis; testis transplantation
资金
- Genome Network Project Japan Science and Technology Agency (CREST)
- Program for Promotion of Basic and Applied Researches for Innovation in Bio orientated Industry
- Ministry of Health Labor, and Welfare
- Senri Life Science Foundation
- Novartis Foundation (Japan) for the Promotion of Science Astellas Foundation for Research on Metabolic Disordors
- Ministry of Education Culture Sports Science and Technology (MEXT)
Spermatogonial stem cells (SSCs) undergo self-renewal divisions to support spermatogenesis Although several in vitro SSC culture systems have been developed, these systems include serum or fibroblast feeders, which complicate SSC self renewal analyses Here, we developed a serum- and feeder-free culture system for long-term propagation of SSCs In addition to the SSC self-renewal factors, including glial cell line derived neurotrophic factor, supplementation with fetuin and lipid-associated molecules was required to drive SSC proliferation in vitro Cultured cells proliferated for at least 6 mo at a rate comparable to that of serum supplemented cultured cells However, germline potential was reduced under serum- and feeder-free conditions, as indicated by a lower SSC frequency after germ cell transplantation Nevertheless, the cultured cells completed spermatogenesis and produced offspring following spermatogo mal transplantation into seminiferous tubules of infertile mice This culture system provides a basic platform for understanding the regulation of SSC fate commitment in vitro and for improving SSC culture medium
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据