期刊
JOURNAL OF CHEMICAL PHYSICS
卷 112, 期 18, 页码 7799-7810出版社
AMER INST PHYSICS
DOI: 10.1063/1.481385
关键词
-
Recent achievements in single molecule detection using near-field optical excitation are presented. By proper control of technology, distinct advantages of near-field optics are exploited: (i) the nanometric excitation/emission volume (10(4)-10(5) nm(3)), which provides high spatial resolution, localization of a single molecule within a few nm, and reduced background; (ii) the sensitivity for single molecule orientation in all three dimensions; (iii) the high local brightness, allowing real-time single molecule detection down to mu s resolution; (iv) the simultaneous colocalization with nanometric surface topography. Real-time quantum jumps between singlet and triplet state of an individual molecule are observed. Distributions for triplet state lifetime and crossing yield are determined. Both triplet state lifetime and crossing yield of a single molecule appear to vary in time, due to the local heterogeneity. Individual dendritic molecules containing a single fluorescent core are investigated. The dendritic assemblies are discriminated from free fluorescent cores on the basis of accurate simultaneous localization of both the fluorescent core and the topography of the surrounding dendritic shell. Intramolecular rotational motion of the fluorescent core is observed. Individual green fluorescent proteins are visualized, both in fluorescence and topography. Photoinduced conformational changes to a nonemissive form of the protein are observed, leading to long dark intervals of several seconds. (C) 2000 American Institute of Physics. [S0021-9606(00)70216-9].
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据