期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 271, 期 2, 页码 418-421出版社
ACADEMIC PRESS INC
DOI: 10.1006/bbrc.2000.2631
关键词
embryonic stem cells; pluripotent stem cells; neural stem cells; neuronal differentiation; cell culture; cryopreservation
资金
- NINDS NIH HHS [NS36455] Funding Source: Medline
Embryonic stem cells (ES cells) are developmentally pluripotent cells isolated from pre-implantation mammalian embryos. In cell culture ES cells can be easily differentiated to generate cultures of neural progenitors. We present a simple method for the cryopreservation of these ES-derived neural progenitors. Cryopreserved neural progenitor stocks can be thawed, expanded with FGF2, and differentiated into functional neurons. This method will facilitate studies using ES-derived neural progenitor cells as a cell culture model system for neural development and differentiation. It will also aid studies designed to test the ability of these progenitor cells to functionally engraft and repair damaged neural tissue. (C) 2000 Academic Press.
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