期刊
EMBO JOURNAL
卷 19, 期 10, 页码 2221-2228出版社
OXFORD UNIV PRESS
DOI: 10.1093/emboj/19.10.2221
关键词
general secretory pathway; pili; protein secretion; secretin; secreton
The secreton (type II secretion) and type IV pilus biogenesis branches of the general secretory pathway id Gram-negative bacteria share many features that suggest a common evolutionary origin. Five components of the secreton, the pseudopilins, are similar to subunits of type IV pill, Here, we report that when the 15 genes encoding the pullulanase secreton of Klebsiella oxytoca were expressed on a high copy number plasmid in Escherichia coli, one pseudopilin, PulG, was assembled into pilus-like bundles. Assembly of the 'secreton pilus' required most but not all of the secreton components that are essential for pullulanase secretion, including some with no known homologues in type IV piliation machineries. Two other pseudopilins, pullulanase and two outer membrane-associated secreton components were not associated with pill. Thus, PulG is probably the major component of the pilus, Expression of a type IV pilin gene, the E. coli K-12 gene ppdD, led to secreton-dependent incorporation of PpdD pilin into pill without diminishing pullulanase secretion. This is the first demonstration that pseudopilins can be assembled into pilus-like structures.
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