期刊
EMBO JOURNAL
卷 19, 期 10, 页码 2193-2203出版社
WILEY
DOI: 10.1093/emboj/19.10.2193
关键词
AP-1; clathrin; mannose 6-phosphate receptor; mouse
The heterotetrameric AP-1 complex is involved in the formation of clathrin-coated vesicles at the trans-Golgi network (TGN) and interacts with sorting signals in the cytoplasmic tails of cargo molecules. Targeted disruption of the mouse mu 1A-adaptin gene causes embryonic lethality at day 13.5. In cells deficient in mu 1A-adaptin the remaining AP-1 adaptins do not bind to the TGN. Polarized epithelial cells are the only cells of mu 1A-adaptin-deficient embryos that show gamma-adaptin binding to membranes, indicating the formation of an epithelial specific AP-1B complex and demonstrating the absence of additional mu 1A homologs. Mannose 6-phosphate receptors are cargo molecules that exit the TGN via AP-l-clathrin-coated vesicles. The steady-state distribution of the mannose 6-phosphate receptors MPR46 and MPR300 in mu 1A-deficient cells is shifted to endosomes at the expense of the TGN, MPR46 fails to recycle back from the endosome to the TGN, indicating that AP-1 is required for retrograde endosome to TGN transport of the receptor.
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