期刊
GENE
卷 249, 期 1-2, 页码 153-160出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-1119(00)00149-9
关键词
myxobacteria; non-ribosomal peptide synthetase; polyketide; polyketide synthase; P450 enzyme
资金
- NCI NIH HHS [1 R43 CA79228-01] Funding Source: Medline
The epothilone biosynthetic gene cluster was isolated from Sorangium cellulosum strain SMP44. The gene cluster contains seven genes and spans approx. 56 kb. The genes encoding the PKS, epoA, epoC, epoD, epoE, and epoF, are divided into nine modules. The EpoB protein is a non-ribosomal peptide synthetase (NRPS) that catalyzes formation of the thiazole found in the epothilones. EpoK is a P450 enzyme responsible for the epoxidation of epothilones C and D to epothilones A and B, respectively. EpoK was expressed in Escherichia coli, and the purified protein was shown to convert epothilone D to epothilone B in vitro. (C) 2000 Elsevier Science B.V. All rights reserved.
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