期刊
ANALYTICA CHIMICA ACTA
卷 413, 期 1-2, 页码 137-145出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0003-2670(00)00788-1
关键词
tris(2,2 '-bipyridyl)ruthenium(III) chemiluminescence; high-performance liquid chromatography; tricyclic antidepressants; imipramine; amitriptyline
A simple, selective and sensitive determination method of tricyclic antidepressants (imipramine, desipramine, amitriptyline, nortriptyline and clomipramine) has been developed using high-performance liquid chromatography (HPLC) with chemiluminescence detection. The method is based on the detection of aliphatic secondary or tertiary amino moieties in antidepressants with post-column tris(2,2'-bipyridyl)ruthenium(III) chemiluminescence. Five antidepressants were separated on a trimethylsilyl (TMS) column using st mixture of 50 mM phosphate buffer (pH 7.0) and acetonitrile (55:45, v/v) as a mobile phase. The eluate was on-line mixed with acidic tris(2,2'-bipyridyl)ruthenium(III) solution and the generated chemiluminescence was detected. The detection limits (signal-to-noise ratio=3) for antidepressants examined were 3.2-33.1 pg (12-105 fmol), for an injection volume of 20 mu l. This HPLC system could sensitively detect other tri- or tetracyclic antidepressants and was successfully applied to the determination of imipramine and amitriptyline in human plasma after purification by two-step liquid-liquid extraction, (C) 2000 Elsevier Science B.V. All rights reserved.
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