期刊
ANALYTICA CHIMICA ACTA
卷 413, 期 1-2, 页码 131-136出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0003-2670(00)00753-4
关键词
glucose; CoTAPC/GOD/Nafion biosensor; LC-ECD; microdialysis sampling
In this paper, microdialysis sampling was combined with liquid chromatography with electrochemical detection (LC-ECD) and glucose in rat brain was determined. A cobalt tetraaminophthalocyanine (CoTAPC)/glucose oxidase (GOD)/Nafion film-coated glassy carbon electrode was used to selectively detect the hydrogen peroxide generated enzymatically in the enzyme reactors, without any interference from electroactive species such as L-ascorbate, mate, proteins. The signal current was linearly related to the glucose concentration in the range of 1.0x10(-6)-5.0x10(-3) mol/l at +0.5 V (versus Ag/AgCl) with a detection limit of 5.0x10(-7) mol/l. To measure glucose in rat brain, microdialysis sampling was employed. The dialysate from the microdialysis system was injected into system. The result was 0.74+/-0.10 mmol/l (n=4). (C) 2000 Elsevier Science B.V. All rights reserved.
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