期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 275, 期 20, 页码 15305-15313出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.275.20.15305
关键词
-
资金
- NIDDK NIH HHS [DK38422, DK51526] Funding Source: Medline
Control of energy metabolism by increases of mitochondrial matrix [Ca2+] ([Ca2+](m)) may represent a fundamental mechanism to meet the ATP demand imposed by heart contractions, but the machinery underlying propagation of [Ca2+] signals from ryanodine receptor Ca2+ release channels (RyR) to the mitochondria remains elusive. Using permeabilized cardiac (H9c2) cells we investigated the cytosolic [Ca2+] ([Ca2+](c)) and [Ca2+](m) signals elicited by activation of RyR, Caffeine, Ca2+, and ryanodine evoked [Ca2+](c) spikes that often appeared as frequency-modulated [Ca2+](c) oscillations in these permeabilized cells. Rapid increases in [Ca2+], and activation of the Ca2+-sensitive mitochondrial dehydrogenases were synchronized to the rising phase of the [Ca2+](c) spikes, The RyR-mediated elevations of global [Ca2+](c) were in the submicromolar range, but the rate of [Ca2+](m) increases was as large as it was in the presence of 30 mu M global [Ca2+](c). Furthermore, RyR-dependent increases of [Ca2+](m) were relatively insensitive to buffering of [Ca2+](c) by EGTA. Therefore, RyR-driven rises of [Ca2+](m) appear to result from large and rapid increases of perimitochondrial [Ca2+]. The falling phase of [Ca2+](c) spikes was followed by a rapid decay of [Ca2+](m). CGP37157 slowed down relaxation of [Ca2+](m) spikes, whereas cyclosporin A had no effect, suggesting that activation of the mitochondrial Ca2+ exchangers accounts for rapid reversal of the [Ca2+](m) response with little contribution from the permeability transition pore. Thus, rapid activation of Ca2+ uptake sites and Ca2+ exchangers evoked by RyR-mediated local [Ca2+](c) signals allow mitochondria to respond rapidly to single [Ca2+](c) spikes in cardiac cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据