期刊
JOURNAL OF CHROMATOGRAPHY B
卷 742, 期 1, 页码 173-183出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(00)00162-6
关键词
lamivudine; zidovudine
资金
- NIAID NIH HHS [AI 33363-07] Funding Source: Medline
- NICHD NIH HHS [P30-HD37260] Funding Source: Medline
- PHS HHS [R01 49381] Funding Source: Medline
A HPLC-MS-MS method was developed and validated to measure lamivudine and zidovudine simultaneously in small volumes of human seminal plasma. Sample preparation was simple and rapid, requiring 25 mu l of sample, the use of isotopically labeled lamivudine and zidovudine as internal standards and ultrafiltration through a molecular mass cut-off membrane. Lamivudine and its internal standard were separated from zidovudine and its internal standard with isocratic HPLC. Detection was carried out using tandem mass spectrometry. This validated method was used to analyze seminal samples obtained from six HIV-positive patients prescribed lamivudine and zidovudine. (C) 2000 Elsevier Science B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据