期刊
CANCER LETTERS
卷 153, 期 1-2, 页码 151-160出版社
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0304-3835(00)00362-1
关键词
nucleophosmin/B23; phosphorylation; cell cycle
类别
The recombinant GST-nucleophosmin/B23 and the truncated mutants were tested for phosphorylation in cell-fret extracts of G(2) and M phases or by purified kinases. Our results indicated that a threonine residue at amino acids (a.a.) 185-240 was phosphorylated by cdc2 kinase during the entry of mitosis while the serine phosphorylation site at the middle acidic portion of the molecule (a.a. 83-152) was phosphorylated by casein kinase Ii during G(2) phase. Our results also showed that there was possibly another serine phosphorylation at site other than the middle portion of nucleophosmin/B23 (a.a. 83-152) during the entry of cells into mitosis. The demonstration of the characteristic changes in phosphorylation of nucleophosmin/B23 during the cell cycle implicates important role of nucleophosmin/B23 in the control of the fate of nucleoli and cell growth. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
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